Journal: EBioMedicine

Article Title: The displacement of the SARS-CoV-2 variant Delta with Omicron: An investigation of hospital admissions and upper respiratory viral loads

doi: 10.1016/j.ebiom.2022.104008

Figure Lengend Snippet: SARS-CoV-2 positivity, COVID-19 hospitalizations, and variants trends November- December 2021. (a) SARS-CoV-2 daily positivity rates between November and December 2021 for both symptomatic and asymptomatic testing. (b) COVID-19 related admissions between November and December 2021. (c) SARS-CoV-2 variant distribution between November and December 2021 relative to the 7 day rolling average positives from Johns Hopkins system. (d) Percent SARS-CoV-2 variants in November and December 2021.

Article Snippet: Specimen preparation, extractions, and sequencing were performed as described previously., Library preparation for this cohort was performed using the NEBNext® ARTIC SARS-CoV-2 Companion Kit (VarSkip Short SARS-CoV-2 # E7660-L).

Techniques: Variant Assay

Journal: EBioMedicine

Article Title: The displacement of the SARS-CoV-2 variant Delta with Omicron: An investigation of hospital admissions and upper respiratory viral loads

doi: 10.1016/j.ebiom.2022.104008

Figure Lengend Snippet: SARS-CoV-2 IgG levels in upper respiratory samples of infected vaccinated patients. Boost, patients with booster dose; Vax, fully vaccinated patients who didn't receive a booster dose. Dashed lines demarcate the limit of borderline and negative ELISA results as specified per assay's package insert. Statistical analyses, one-way ANOVA. Total numbers per group are shown on top of each scatter.

Article Snippet: Specimen preparation, extractions, and sequencing were performed as described previously., Library preparation for this cohort was performed using the NEBNext® ARTIC SARS-CoV-2 Companion Kit (VarSkip Short SARS-CoV-2 # E7660-L).

Techniques: Infection, Enzyme-linked Immunosorbent Assay

Journal: medRxiv

Article Title: One-Step Rapid Quantification of Serum Neutralizing Antibody after COVID-19 Vaccination by a High-Throughput Nanoplasmonic Sensor Platform

doi: 10.1101/2021.04.21.21255838

Figure Lengend Snippet: Label-free detection of the total SARS-CoV-2 NAs with colloidal GNP-enhanced NanoPISA. (A) Schematic diagram of the NanoPISA for the determination of SARS-CoV-2 Nas with colloidal GNP enhancement. (B) The differential spectra of SARS-CoV-2 NAs at a concentration range from 8 to 512 ng/mL at 590 nm and 610 nm resonant wavelengths. (C) Dynamic binding curves of SARS-CoV-2 NAs at a concentration range from 8 to 512 ng/mL at 610 nm resonant wavelength. (D) Standard curve for SARS-CoV-2 NAs (R = 0.992).

Article Snippet: In addition, the detection of the SARS-CoV-2 NAs by NanoPISA were validated by SARS-CoV-2 Antibody Assay ELISA kit (XG100H2, AbMax Biotechnology Co., Ltd.) and SARS-CoV-2 surrogate virus neutralizion ELISA kit (L00847-A, Nanjing GenScript Biotech Co., Ltd.).

Techniques: Concentration Assay, Binding Assay

Journal: medRxiv

Article Title: One-Step Rapid Quantification of Serum Neutralizing Antibody after COVID-19 Vaccination by a High-Throughput Nanoplasmonic Sensor Platform

doi: 10.1101/2021.04.21.21255838

Figure Lengend Snippet: Label-free detection of the total SARS-CoV-2 NAs with NHGNP enhancement based on the NanoPISA platform. (A) Schematic diagram of the nanoplasmonic resonance sensor for the determination of SARS-CoV-2 NAs with NHGNP enhancement. (B) The differential spectra of SARS-CoV-2 NAs at different concentrations (0–125 ng/mL) at 600 nm and 650 nm resonant wavelengths. (C) Dynamic binding curves of SARS-CoV-2 NAs at different concentrations over the range from 0 to 125 ng/mL. (D) Standard curve of SARS-CoV-2 NA detection by NHGNP-labeled S-RBD (R2 = 0.998, LOD = 0.1 ng/mL).

Article Snippet: In addition, the detection of the SARS-CoV-2 NAs by NanoPISA were validated by SARS-CoV-2 Antibody Assay ELISA kit (XG100H2, AbMax Biotechnology Co., Ltd.) and SARS-CoV-2 surrogate virus neutralizion ELISA kit (L00847-A, Nanjing GenScript Biotech Co., Ltd.).

Techniques: Binding Assay, Labeling

Journal: Diagnostics

Article Title: Nucleic Acid and Immunological Diagnostics for SARS-CoV-2: Processes, Platforms and Pitfalls

doi: 10.3390/diagnostics10110866

Figure Lengend Snippet: List of major commercial Reverse Transcription quantitative Polymerase Chain Reaction (RT-qPCR) assays available for Severe Acute Respiratory Syndrome CoronaVirus 2 (SARS-CoV-2) diagnosis.

Article Snippet: 19 , UBI SARS-CoV-2 ELISA , indirect ELISA , Not provided , , NA , NA , CE mark 4/2020 , Microplate reader , United Biomedical.

Techniques: Reverse Transcription, Real-time Polymerase Chain Reaction, Biomarker Discovery, Control, Multiplex Assay, Quantitative RT-PCR, Diagnostic Assay, Extraction

Journal: Diagnostics

Article Title: Nucleic Acid and Immunological Diagnostics for SARS-CoV-2: Processes, Platforms and Pitfalls

doi: 10.3390/diagnostics10110866

Figure Lengend Snippet: Immunological tests available globally for detection of COVID-19.

Article Snippet: 19 , UBI SARS-CoV-2 ELISA , indirect ELISA , Not provided , , NA , NA , CE mark 4/2020 , Microplate reader , United Biomedical.

Techniques: Enzyme-linked Immunosorbent Assay, Indirect ELISA, Magnetic Beads, Luminex, Fluorescence, Surround Optical-fiber Immunoassay, Virus, Diagnostic Assay, Microarray